60Co gamma ray induced mutants of cowpea and assessment of genetic variability by SCoT marker

نویسندگان

چکیده

Mutagenesis is a well-known technique for introducing new variants into crop plants. In the present study, M2 populations were generated in cowpea (Vigna unguiculata (L.) Walp.) variety CO7 using gamma irradiation. The progeny used to investigate effectiveness of irradiation doses and examined agronomic traits. genomic variation mutants their parents was analysed five SCoT markers. Marker analysis revealed total 87 amplicons among these, 20 showed polymorphism. highest numbers observed at SCoT10 (39), while lowest number produced by SCoT09 (07). percentage polymorphism ranged from 18.18% 28.57%, with an average 21.12%. Polymorphic information content (PIC) values 0.197 0.345. Analysis Molecular Variation (AMOVA) 12% 88% between genotypes within respectively. constructions 4 clusters identified through Unweighted Pair Group Method Arithmetic Mean (UPGMA) dendrogram tree based on genetic distance deduced marker analysis. relatedness parent Principal Coordinate (PCoA) two main groups. study concludes that variability induced inherited next generations. This research investigation supports alters growth yield traits, which helpful generating improvement.

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ژورنال

عنوان ژورنال: Plant science today

سال: 2022

ISSN: ['2348-1900']

DOI: https://doi.org/10.14719/pst.1623