60Co gamma ray induced mutants of cowpea and assessment of genetic variability by SCoT marker
نویسندگان
چکیده
Mutagenesis is a well-known technique for introducing new variants into crop plants. In the present study, M2 populations were generated in cowpea (Vigna unguiculata (L.) Walp.) variety CO7 using gamma irradiation. The progeny used to investigate effectiveness of irradiation doses and examined agronomic traits. genomic variation mutants their parents was analysed five SCoT markers. Marker analysis revealed total 87 amplicons among these, 20 showed polymorphism. highest numbers observed at SCoT10 (39), while lowest number produced by SCoT09 (07). percentage polymorphism ranged from 18.18% 28.57%, with an average 21.12%. Polymorphic information content (PIC) values 0.197 0.345. Analysis Molecular Variation (AMOVA) 12% 88% between genotypes within respectively. constructions 4 clusters identified through Unweighted Pair Group Method Arithmetic Mean (UPGMA) dendrogram tree based on genetic distance deduced marker analysis. relatedness parent Principal Coordinate (PCoA) two main groups. study concludes that variability induced inherited next generations. This research investigation supports alters growth yield traits, which helpful generating improvement.
منابع مشابه
Molecular and morphological assessment of genetic variability induced by gamma radiation in canola
Mutation induction is considered as an effective way to enrich plant genetic variation, particularly for traits with a very low level of genetic variation. This research was conducted to assess genetic variation induced by gamma radiation in M2 and M3 mutant lines of canola (Brassica napus L.) by SSR and morphological characteristics and to identify useful mutants in terms of agronomic traits. ...
متن کاملmolecular and morphological assessment of genetic variability induced by gamma radiation in canola
mutation induction is considered as an effective way to enrich plant genetic variation, particularly fortraits with a very low level of genetic variation. this research was conducted to assess genetic variationinduced by gamma radiation in m2 and m3 mutant lines of canola (brassica napus l.) by ssr and morphologicalcharacteristics and to identify useful mutants in terms of agronomic traits. six...
متن کاملInvestigation of Mutation in Chitinase Gene in Gamma Radiated Mutants of Trichoderma Harzianum by STS Marker
Effects of induced mutation via gamma irradiated with 250 Gy dose (in Nuclear Agriculture Research School- Nuclear Science and Technology Research Institute) on Trichoderma harzianum conidiaon chitinase activity by STS molecular marker have been evaluated in this study. Among 20 irradiated mutants which selected via improved antagonistic capability against Rhizoctonia sola...
متن کاملInvestigation of Mutation in Chitinase Gene in Gamma Radiated Mutants of Trichoderma Harzianum by STS Marker
Effects of induced mutation via gamma irradiated with 250 Gy dose (in Nuclear Agriculture Research School- Nuclear Science and Technology Research Institute) on Trichoderma harzianum conidiaon chitinase activity by STS molecular marker have been evaluated in this study. Among 20 irradiated mutants which selected via improved antagonistic capability against Rhizoctonia sola...
متن کاملGenetic variability and identification of markers associated with germination parameters in gamma-irradiation induced mutants of sunflower under water stress condition
The objectives of the present research were to evaluate the variability induced by gamma-irradiation among a population of M8 sunflower mutant lines and to identify molecular markers associated with different seed germination traits. Experiments were carried out under well watered and water-stressed conditions using a randomized blocks design, with three replications. The studied traits consist...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant science today
سال: 2022
ISSN: ['2348-1900']
DOI: https://doi.org/10.14719/pst.1623